GNU Emacs Lisp Reference Manual
kill-region is the usual subroutine for killing text. Any command that calls this function is a ``kill command'' (and should probably have `kill' in its name). kill-region puts the newly killed text in a new element at the beginning of the kill ring or adds it to the most recent element. It uses the last-command variable to determine whether the previous command was a kill command, and if so appends the killed text to the most recent entry.
nil. In an interactive call, start and end are point and the mark.
If the buffer is read-only, kill-region modifies the kill ring just the same, then signals an error without modifying the buffer. This is convenient because it lets the user use all the kill commands to copy text into the kill ring from a read-only buffer.
nil. It also indicates the extent of the text copied by moving the cursor momentarily, or by displaying a message in the echo area. The command does not set this-command to kill-region, so a subsequent kill command does not append to the same kill ring entry.
Don't call copy-region-as-kill in Lisp programs unless you aim to support Emacs 18. For Emacs 19, it is better to use kill-new or kill-append instead. See Low-Level Kill Ring.