read.exprSet package:Biobase R Documentation _R_e_a_d _E_x_p_e_r_i_m_e_n_t_a_l _D_a_t_a _a_n_d _i_n_t_o _a_n '_e_x_p_r_S_e_t' _O_b_j_e_c_t _D_e_s_c_r_i_p_t_i_o_n: An exprSet object has a slot named 'exprs' for experimental data. 'read.exprSet' reads the experimental data stored as a text file and then generates an exprSet object using the data. Data for other slots can be also read in as options. _U_s_a_g_e: read.exprSet(exprs, se.exprs, phenoData, annotation, description, notes, seps = "\t") _A_r_g_u_m_e_n_t_s: exprs: 'exprs' a character string for the name of the text file that stores the experimental data to be read in. The text file is assumed to be a delimited file with observed expression levels arranged in rows and columns. The first row of the file is sample names and the first column is gene names/ids. The file will be read in using read.table with header set to TRUE se.exprs: 'se.exprs' a character stirng for the name of a delimited text file contains standard error estimates for the estimated expression in exprs. The file is of the same dimensions as 'exprs' phenoData: 'phenoData' a character string for the name of an optional file containing information for 'pData' slot of a phenoData object to be returned. If missing a default phenoData object with one covariate ranging from 1 to the number of columns of 'exprs' will be provided annotation: 'annotation' a character string for the name of an optional text file that contains a string identifying the annotation that may be used for the 'exprSet' instance description: 'description' a character string for the name of an optional file containing MIAME information notes: 'notes' a character string for the name of an optional text file containing explanatory text seps: 'seps' a vector of character strings defining the delimitors used by 'exprs' and 'se.exprs'. The length of 'seps' can be 1 when both file have the same delimiter or 'se.exprs' is missing or 2 other wise. The delimitor for 'exprs' is expected to be the first element _D_e_t_a_i_l_s: If not missing, 'phenoData' will be read in using 'read.phenoData' and description using 'read.MIAME'. The help files of the two functions contain detailed descriptions of the two functions. _V_a_l_u_e: This function returns an 'exprSet' object. _N_o_t_e: This function is part of the Bioconductor project at Dana-Farber Cancer Institute to provide bioinformatics functionalities through R _A_u_t_h_o_r(_s): Jianhua Zhang _S_e_e _A_l_s_o: 'exprSet', 'phenoData', 'MIAME', 'read.phenoData', 'read.MIAME' _E_x_a_m_p_l_e_s: # Creating a test data testFile <- tempfile() data(geneData) write.table(geneData, testFile, quote = FALSE, sep = "\t", row.names = TRUE, col.names = TRUE) # Create an exprSet object using textFile eSet <- read.exprSet(testFile) eSet